Bi-transgenic models

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Mouse models having two transgenes are called bi transgenic. To test the cooperation of  oncogenes, Tim Stewert and organization made the primary bi-transgenic mouse fashions in 1987, MMTV-Myc and MMTV- Ras mice had been crossed with a ensuing acceleration in tumorigenesis. Expression of TGFβ within the breast cancer cells of MMTV-ErbB2; MMTV-TGFβ double-transgenic mice can result in higher ranges of circulating tumor cells and lung metastasis. Ras gene can be combined with rtTA (opposite tetracycline transactivator) to generate bi-transgenic inducible mouse model thru tetracycline-controlled transcriptional activation e.G. Mice carrying TetO-KrasG12D (TOR) and MMTV-rtTA (MTB), comes with the transgene expressing the opposite tetracycline transactivator (rtTA) in mammary epithelial cells.